Composite

Part:BBa_K953000:Design

Designed by: Ryan Kenny   Group: iGEM12_Macquarie_Australia   (2012-09-20)


Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GC rich regions, which formed hairpin loops in synthesis, were removed. Restriction sites were removed to conform to biobrick standards.


Source

The part was codon optimsed (http://www.bioinformatics.org/sms2/rev_trans.html) from Synechocystis and assembled using Gibson Assembly. Select GC rich regions were removed.

References